Centrifugal microfluidic system for primary amplification and secondary real-time PCR.
نویسندگان
چکیده
Pre-amplification is a basis for numerous polymerase chain reaction (PCR) protocols but bears severe contamination risks due to handling of high-copy DNA samples. Therefore we developed a self-contained centrifugal microfluidic system comprising pre-stored reagents; it enables pre-amplification of specific DNA sequences prior to automated aliquoting and real-time PCR in a modified commercial thermocycler.
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ورودعنوان ژورنال:
- Lab on a chip
دوره 10 23 شماره
صفحات -
تاریخ انتشار 2010